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    plexon inc offline sorter, v4.3.1
    Offline Sorter, V4.3.1, supplied by plexon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/offline+sorter%2C+v4%2E3%2E1/offline+sorter++v4+3+1/pm30355486-210-188-189
    Average 90 stars, based on 1 article reviews
    offline sorter, v4.3.1 - by Bioz Stars, 2026-09
    90/100 stars

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    Reverse Transcription:

    Article Title: Human Huntington's Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ki67 Vector Laboratories Cat# VP-K451, RRID:AB_2314701 Nestin Abcam Cat# ABD69, RRID:AB_2744681 SSEA4 Stemgent Cat# EB09006, RRID:AB_2179791 FOXG1 Abcam Cat# ab18259, RRID:AB_732415 ZO1 Invitrogen Cat# 61-7300, RRID:AB_138452 DCX Aves labs Cat# DCX, RRID:AB_2313540 Beta-Tubulin III/TUJ1 Sigma-Aldrich Cat# T8660, RRID:AB_477590 Map2ab Sigma Cat# M1406, RRID:AB_477171 TBR2 Abcam Cat# ab23345, RRID:AB_778267 TBR1 Abcam Cat# ab31940, RRID:AB_2200219 Ctip2 Abcam Cat# ab18465, RRID:AB_2064130 SatB2 Abcam Cat# ab51502, RRID:AB_882455 GFAP Aves labs Cat# LS-C51002-100, RRID:AB_1220403 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21202, RRID:AB_141607 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31571, RRID:AB_162542 Goat anti-Mouse IgG (H+L) Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-11005, RRID:AB_141372 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21206, RRID:AB_141708 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-21207, RRID:AB_141637 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31573, RRID:AB_2536183 Chemicals, Peptides, and Recombinant Proteins Matrigel Corning 354230 mTeSR StemCell Technologies, Inc. 5850 EZ tool Life Technologies 23181-010 rock inhibitor (Y27632) Tocris 1254 DMEM:F12 Life Technologies 11330 Neurobasal Life Technologies 21103-049 Insulin Sigma-Aldrich I9278-5ML NEAA Life Technologies 11140-050 L-Glutamine Life Technologies 25030-081 antibiotic/antimycotic Life Technologies 15240-062 N2 Life Technologies 17502048 B27 Life Technologies 17504044 SB43142 Tocris 1614 LDN193189 Selleck S2618 TrypLE Thermofisher 12604013 FGF2 Peprotech 100-18B paraformaldehyde EMS 1224SK-SP (Continued on next page) Cell Reports 25, 1081–1096.e1–e6, October 23, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER 40,6-diamidino-2-phenylindole (DAPI) molecular probes D3571 1mm OD thin wall glass capillaries Fisher Scientific 22-362566 Trizol Thermo Fischer Scientific 155596026 choloroform Sigma-Aldrich C2432-25ML RNeasy column QIAGEN 74106 RNase-free DNase QIAGEN 79254 reverse transcriptase Invitrogen LS18064022 Agencourt AMPure XP beads Beckman Coulter A63880 Critical Commercial Assays 48 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-48 96 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-96 TruSeq Stranded mRNA library preparation kit Illumina 20020594 Deposited Data Raw data and statistical analysis (Mendeley Data) This paper https://doi.org/10.17632/y275mydz78.1 RNA sequencing data This paper GEO: GSE109534 Experimental Models: Cell Lines CS97iHD180n; male, 6 years iPSC Core (CSMC) CS97iHD180n CS09iHD109n; female, age unknown iPSC Core (CSMC) CS09iHD109n CS77iHD77n; male, age unknown iPSC Core (CSMC) CS77iHD77n CS83iCTR33n; female, 21 years iPSC Core (CSMC) CS83iCTR33n CS00iCTR21n; male, 6 years iPSC Core (CSMC) CS00iCTR21n CS25iCTR18n; male, 76 years iPSC Core (CSMC) CS25iCTR18n Experimental Models: Organisms/Strains Qubit fluorometer ThermoFisher Scientific Qubit fluorometer 2100 Bioanalyzer Agilent Technologies 2100 Bioanalyzer Software and Algorithms Neuroleucida 11.07.3 MBF Biosciences v11.07.3 Neuroexplorer 360 11.09 MBF Biosciences v11.09 Maestro Axis software version 2.4.2 Axion Biosystems v2.4.2 Plexon Offline Sorter, v4.3.1 Plexon v4.3.1 STAR (version 2.5.0) / RSEM (version 1.2.25) http://code.google.com/p/rna-star/ Open source DESeq2 Bioconductor package version 1.10.1 in R version 3.2.2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Open source Bioconductor g-plots package (version 2.14.2) in R https://www.bioconductor.org/install/ Open source DAVID Bioinformatics database (version 6.8) https://david.ncifcrf.gov/ Open source WGCNA was performed using its package in R https://cran.r-project.org/web/ packages/WGCNA/index.html Open source SAS v9.4 software SAS Institute Inc. v9.4 ..

    Microarray:

    Article Title: Human Huntington's Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ki67 Vector Laboratories Cat# VP-K451, RRID:AB_2314701 Nestin Abcam Cat# ABD69, RRID:AB_2744681 SSEA4 Stemgent Cat# EB09006, RRID:AB_2179791 FOXG1 Abcam Cat# ab18259, RRID:AB_732415 ZO1 Invitrogen Cat# 61-7300, RRID:AB_138452 DCX Aves labs Cat# DCX, RRID:AB_2313540 Beta-Tubulin III/TUJ1 Sigma-Aldrich Cat# T8660, RRID:AB_477590 Map2ab Sigma Cat# M1406, RRID:AB_477171 TBR2 Abcam Cat# ab23345, RRID:AB_778267 TBR1 Abcam Cat# ab31940, RRID:AB_2200219 Ctip2 Abcam Cat# ab18465, RRID:AB_2064130 SatB2 Abcam Cat# ab51502, RRID:AB_882455 GFAP Aves labs Cat# LS-C51002-100, RRID:AB_1220403 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21202, RRID:AB_141607 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31571, RRID:AB_162542 Goat anti-Mouse IgG (H+L) Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-11005, RRID:AB_141372 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21206, RRID:AB_141708 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-21207, RRID:AB_141637 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31573, RRID:AB_2536183 Chemicals, Peptides, and Recombinant Proteins Matrigel Corning 354230 mTeSR StemCell Technologies, Inc. 5850 EZ tool Life Technologies 23181-010 rock inhibitor (Y27632) Tocris 1254 DMEM:F12 Life Technologies 11330 Neurobasal Life Technologies 21103-049 Insulin Sigma-Aldrich I9278-5ML NEAA Life Technologies 11140-050 L-Glutamine Life Technologies 25030-081 antibiotic/antimycotic Life Technologies 15240-062 N2 Life Technologies 17502048 B27 Life Technologies 17504044 SB43142 Tocris 1614 LDN193189 Selleck S2618 TrypLE Thermofisher 12604013 FGF2 Peprotech 100-18B paraformaldehyde EMS 1224SK-SP (Continued on next page) Cell Reports 25, 1081–1096.e1–e6, October 23, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER 40,6-diamidino-2-phenylindole (DAPI) molecular probes D3571 1mm OD thin wall glass capillaries Fisher Scientific 22-362566 Trizol Thermo Fischer Scientific 155596026 choloroform Sigma-Aldrich C2432-25ML RNeasy column QIAGEN 74106 RNase-free DNase QIAGEN 79254 reverse transcriptase Invitrogen LS18064022 Agencourt AMPure XP beads Beckman Coulter A63880 Critical Commercial Assays 48 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-48 96 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-96 TruSeq Stranded mRNA library preparation kit Illumina 20020594 Deposited Data Raw data and statistical analysis (Mendeley Data) This paper https://doi.org/10.17632/y275mydz78.1 RNA sequencing data This paper GEO: GSE109534 Experimental Models: Cell Lines CS97iHD180n; male, 6 years iPSC Core (CSMC) CS97iHD180n CS09iHD109n; female, age unknown iPSC Core (CSMC) CS09iHD109n CS77iHD77n; male, age unknown iPSC Core (CSMC) CS77iHD77n CS83iCTR33n; female, 21 years iPSC Core (CSMC) CS83iCTR33n CS00iCTR21n; male, 6 years iPSC Core (CSMC) CS00iCTR21n CS25iCTR18n; male, 76 years iPSC Core (CSMC) CS25iCTR18n Experimental Models: Organisms/Strains Qubit fluorometer ThermoFisher Scientific Qubit fluorometer 2100 Bioanalyzer Agilent Technologies 2100 Bioanalyzer Software and Algorithms Neuroleucida 11.07.3 MBF Biosciences v11.07.3 Neuroexplorer 360 11.09 MBF Biosciences v11.09 Maestro Axis software version 2.4.2 Axion Biosystems v2.4.2 Plexon Offline Sorter, v4.3.1 Plexon v4.3.1 STAR (version 2.5.0) / RSEM (version 1.2.25) http://code.google.com/p/rna-star/ Open source DESeq2 Bioconductor package version 1.10.1 in R version 3.2.2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Open source Bioconductor g-plots package (version 2.14.2) in R https://www.bioconductor.org/install/ Open source DAVID Bioinformatics database (version 6.8) https://david.ncifcrf.gov/ Open source WGCNA was performed using its package in R https://cran.r-project.org/web/ packages/WGCNA/index.html Open source SAS v9.4 software SAS Institute Inc. v9.4 ..

    Microelectrode Array:

    Article Title: Human Huntington's Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ki67 Vector Laboratories Cat# VP-K451, RRID:AB_2314701 Nestin Abcam Cat# ABD69, RRID:AB_2744681 SSEA4 Stemgent Cat# EB09006, RRID:AB_2179791 FOXG1 Abcam Cat# ab18259, RRID:AB_732415 ZO1 Invitrogen Cat# 61-7300, RRID:AB_138452 DCX Aves labs Cat# DCX, RRID:AB_2313540 Beta-Tubulin III/TUJ1 Sigma-Aldrich Cat# T8660, RRID:AB_477590 Map2ab Sigma Cat# M1406, RRID:AB_477171 TBR2 Abcam Cat# ab23345, RRID:AB_778267 TBR1 Abcam Cat# ab31940, RRID:AB_2200219 Ctip2 Abcam Cat# ab18465, RRID:AB_2064130 SatB2 Abcam Cat# ab51502, RRID:AB_882455 GFAP Aves labs Cat# LS-C51002-100, RRID:AB_1220403 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21202, RRID:AB_141607 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31571, RRID:AB_162542 Goat anti-Mouse IgG (H+L) Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-11005, RRID:AB_141372 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21206, RRID:AB_141708 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-21207, RRID:AB_141637 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31573, RRID:AB_2536183 Chemicals, Peptides, and Recombinant Proteins Matrigel Corning 354230 mTeSR StemCell Technologies, Inc. 5850 EZ tool Life Technologies 23181-010 rock inhibitor (Y27632) Tocris 1254 DMEM:F12 Life Technologies 11330 Neurobasal Life Technologies 21103-049 Insulin Sigma-Aldrich I9278-5ML NEAA Life Technologies 11140-050 L-Glutamine Life Technologies 25030-081 antibiotic/antimycotic Life Technologies 15240-062 N2 Life Technologies 17502048 B27 Life Technologies 17504044 SB43142 Tocris 1614 LDN193189 Selleck S2618 TrypLE Thermofisher 12604013 FGF2 Peprotech 100-18B paraformaldehyde EMS 1224SK-SP (Continued on next page) Cell Reports 25, 1081–1096.e1–e6, October 23, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER 40,6-diamidino-2-phenylindole (DAPI) molecular probes D3571 1mm OD thin wall glass capillaries Fisher Scientific 22-362566 Trizol Thermo Fischer Scientific 155596026 choloroform Sigma-Aldrich C2432-25ML RNeasy column QIAGEN 74106 RNase-free DNase QIAGEN 79254 reverse transcriptase Invitrogen LS18064022 Agencourt AMPure XP beads Beckman Coulter A63880 Critical Commercial Assays 48 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-48 96 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-96 TruSeq Stranded mRNA library preparation kit Illumina 20020594 Deposited Data Raw data and statistical analysis (Mendeley Data) This paper https://doi.org/10.17632/y275mydz78.1 RNA sequencing data This paper GEO: GSE109534 Experimental Models: Cell Lines CS97iHD180n; male, 6 years iPSC Core (CSMC) CS97iHD180n CS09iHD109n; female, age unknown iPSC Core (CSMC) CS09iHD109n CS77iHD77n; male, age unknown iPSC Core (CSMC) CS77iHD77n CS83iCTR33n; female, 21 years iPSC Core (CSMC) CS83iCTR33n CS00iCTR21n; male, 6 years iPSC Core (CSMC) CS00iCTR21n CS25iCTR18n; male, 76 years iPSC Core (CSMC) CS25iCTR18n Experimental Models: Organisms/Strains Qubit fluorometer ThermoFisher Scientific Qubit fluorometer 2100 Bioanalyzer Agilent Technologies 2100 Bioanalyzer Software and Algorithms Neuroleucida 11.07.3 MBF Biosciences v11.07.3 Neuroexplorer 360 11.09 MBF Biosciences v11.09 Maestro Axis software version 2.4.2 Axion Biosystems v2.4.2 Plexon Offline Sorter, v4.3.1 Plexon v4.3.1 STAR (version 2.5.0) / RSEM (version 1.2.25) http://code.google.com/p/rna-star/ Open source DESeq2 Bioconductor package version 1.10.1 in R version 3.2.2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Open source Bioconductor g-plots package (version 2.14.2) in R https://www.bioconductor.org/install/ Open source DAVID Bioinformatics database (version 6.8) https://david.ncifcrf.gov/ Open source WGCNA was performed using its package in R https://cran.r-project.org/web/ packages/WGCNA/index.html Open source SAS v9.4 software SAS Institute Inc. v9.4 ..

    RNA Sequencing:

    Article Title: Human Huntington's Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ki67 Vector Laboratories Cat# VP-K451, RRID:AB_2314701 Nestin Abcam Cat# ABD69, RRID:AB_2744681 SSEA4 Stemgent Cat# EB09006, RRID:AB_2179791 FOXG1 Abcam Cat# ab18259, RRID:AB_732415 ZO1 Invitrogen Cat# 61-7300, RRID:AB_138452 DCX Aves labs Cat# DCX, RRID:AB_2313540 Beta-Tubulin III/TUJ1 Sigma-Aldrich Cat# T8660, RRID:AB_477590 Map2ab Sigma Cat# M1406, RRID:AB_477171 TBR2 Abcam Cat# ab23345, RRID:AB_778267 TBR1 Abcam Cat# ab31940, RRID:AB_2200219 Ctip2 Abcam Cat# ab18465, RRID:AB_2064130 SatB2 Abcam Cat# ab51502, RRID:AB_882455 GFAP Aves labs Cat# LS-C51002-100, RRID:AB_1220403 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21202, RRID:AB_141607 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31571, RRID:AB_162542 Goat anti-Mouse IgG (H+L) Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-11005, RRID:AB_141372 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21206, RRID:AB_141708 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-21207, RRID:AB_141637 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31573, RRID:AB_2536183 Chemicals, Peptides, and Recombinant Proteins Matrigel Corning 354230 mTeSR StemCell Technologies, Inc. 5850 EZ tool Life Technologies 23181-010 rock inhibitor (Y27632) Tocris 1254 DMEM:F12 Life Technologies 11330 Neurobasal Life Technologies 21103-049 Insulin Sigma-Aldrich I9278-5ML NEAA Life Technologies 11140-050 L-Glutamine Life Technologies 25030-081 antibiotic/antimycotic Life Technologies 15240-062 N2 Life Technologies 17502048 B27 Life Technologies 17504044 SB43142 Tocris 1614 LDN193189 Selleck S2618 TrypLE Thermofisher 12604013 FGF2 Peprotech 100-18B paraformaldehyde EMS 1224SK-SP (Continued on next page) Cell Reports 25, 1081–1096.e1–e6, October 23, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER 40,6-diamidino-2-phenylindole (DAPI) molecular probes D3571 1mm OD thin wall glass capillaries Fisher Scientific 22-362566 Trizol Thermo Fischer Scientific 155596026 choloroform Sigma-Aldrich C2432-25ML RNeasy column QIAGEN 74106 RNase-free DNase QIAGEN 79254 reverse transcriptase Invitrogen LS18064022 Agencourt AMPure XP beads Beckman Coulter A63880 Critical Commercial Assays 48 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-48 96 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-96 TruSeq Stranded mRNA library preparation kit Illumina 20020594 Deposited Data Raw data and statistical analysis (Mendeley Data) This paper https://doi.org/10.17632/y275mydz78.1 RNA sequencing data This paper GEO: GSE109534 Experimental Models: Cell Lines CS97iHD180n; male, 6 years iPSC Core (CSMC) CS97iHD180n CS09iHD109n; female, age unknown iPSC Core (CSMC) CS09iHD109n CS77iHD77n; male, age unknown iPSC Core (CSMC) CS77iHD77n CS83iCTR33n; female, 21 years iPSC Core (CSMC) CS83iCTR33n CS00iCTR21n; male, 6 years iPSC Core (CSMC) CS00iCTR21n CS25iCTR18n; male, 76 years iPSC Core (CSMC) CS25iCTR18n Experimental Models: Organisms/Strains Qubit fluorometer ThermoFisher Scientific Qubit fluorometer 2100 Bioanalyzer Agilent Technologies 2100 Bioanalyzer Software and Algorithms Neuroleucida 11.07.3 MBF Biosciences v11.07.3 Neuroexplorer 360 11.09 MBF Biosciences v11.09 Maestro Axis software version 2.4.2 Axion Biosystems v2.4.2 Plexon Offline Sorter, v4.3.1 Plexon v4.3.1 STAR (version 2.5.0) / RSEM (version 1.2.25) http://code.google.com/p/rna-star/ Open source DESeq2 Bioconductor package version 1.10.1 in R version 3.2.2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Open source Bioconductor g-plots package (version 2.14.2) in R https://www.bioconductor.org/install/ Open source DAVID Bioinformatics database (version 6.8) https://david.ncifcrf.gov/ Open source WGCNA was performed using its package in R https://cran.r-project.org/web/ packages/WGCNA/index.html Open source SAS v9.4 software SAS Institute Inc. v9.4 ..

    Software:

    Article Title: Human Huntington's Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ki67 Vector Laboratories Cat# VP-K451, RRID:AB_2314701 Nestin Abcam Cat# ABD69, RRID:AB_2744681 SSEA4 Stemgent Cat# EB09006, RRID:AB_2179791 FOXG1 Abcam Cat# ab18259, RRID:AB_732415 ZO1 Invitrogen Cat# 61-7300, RRID:AB_138452 DCX Aves labs Cat# DCX, RRID:AB_2313540 Beta-Tubulin III/TUJ1 Sigma-Aldrich Cat# T8660, RRID:AB_477590 Map2ab Sigma Cat# M1406, RRID:AB_477171 TBR2 Abcam Cat# ab23345, RRID:AB_778267 TBR1 Abcam Cat# ab31940, RRID:AB_2200219 Ctip2 Abcam Cat# ab18465, RRID:AB_2064130 SatB2 Abcam Cat# ab51502, RRID:AB_882455 GFAP Aves labs Cat# LS-C51002-100, RRID:AB_1220403 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21202, RRID:AB_141607 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31571, RRID:AB_162542 Goat anti-Mouse IgG (H+L) Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-11005, RRID:AB_141372 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 488 Life Technologies Cat# A-21206, RRID:AB_141708 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 594 Life Technologies Cat# A-21207, RRID:AB_141637 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647 Life Technologies Cat# A-31573, RRID:AB_2536183 Chemicals, Peptides, and Recombinant Proteins Matrigel Corning 354230 mTeSR StemCell Technologies, Inc. 5850 EZ tool Life Technologies 23181-010 rock inhibitor (Y27632) Tocris 1254 DMEM:F12 Life Technologies 11330 Neurobasal Life Technologies 21103-049 Insulin Sigma-Aldrich I9278-5ML NEAA Life Technologies 11140-050 L-Glutamine Life Technologies 25030-081 antibiotic/antimycotic Life Technologies 15240-062 N2 Life Technologies 17502048 B27 Life Technologies 17504044 SB43142 Tocris 1614 LDN193189 Selleck S2618 TrypLE Thermofisher 12604013 FGF2 Peprotech 100-18B paraformaldehyde EMS 1224SK-SP (Continued on next page) Cell Reports 25, 1081–1096.e1–e6, October 23, 2018 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER 40,6-diamidino-2-phenylindole (DAPI) molecular probes D3571 1mm OD thin wall glass capillaries Fisher Scientific 22-362566 Trizol Thermo Fischer Scientific 155596026 choloroform Sigma-Aldrich C2432-25ML RNeasy column QIAGEN 74106 RNase-free DNase QIAGEN 79254 reverse transcriptase Invitrogen LS18064022 Agencourt AMPure XP beads Beckman Coulter A63880 Critical Commercial Assays 48 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-48 96 well microarray electrode array (MEA) plates Axion Biosystems M768-KAP-96 TruSeq Stranded mRNA library preparation kit Illumina 20020594 Deposited Data Raw data and statistical analysis (Mendeley Data) This paper https://doi.org/10.17632/y275mydz78.1 RNA sequencing data This paper GEO: GSE109534 Experimental Models: Cell Lines CS97iHD180n; male, 6 years iPSC Core (CSMC) CS97iHD180n CS09iHD109n; female, age unknown iPSC Core (CSMC) CS09iHD109n CS77iHD77n; male, age unknown iPSC Core (CSMC) CS77iHD77n CS83iCTR33n; female, 21 years iPSC Core (CSMC) CS83iCTR33n CS00iCTR21n; male, 6 years iPSC Core (CSMC) CS00iCTR21n CS25iCTR18n; male, 76 years iPSC Core (CSMC) CS25iCTR18n Experimental Models: Organisms/Strains Qubit fluorometer ThermoFisher Scientific Qubit fluorometer 2100 Bioanalyzer Agilent Technologies 2100 Bioanalyzer Software and Algorithms Neuroleucida 11.07.3 MBF Biosciences v11.07.3 Neuroexplorer 360 11.09 MBF Biosciences v11.09 Maestro Axis software version 2.4.2 Axion Biosystems v2.4.2 Plexon Offline Sorter, v4.3.1 Plexon v4.3.1 STAR (version 2.5.0) / RSEM (version 1.2.25) http://code.google.com/p/rna-star/ Open source DESeq2 Bioconductor package version 1.10.1 in R version 3.2.2 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Open source Bioconductor g-plots package (version 2.14.2) in R https://www.bioconductor.org/install/ Open source DAVID Bioinformatics database (version 6.8) https://david.ncifcrf.gov/ Open source WGCNA was performed using its package in R https://cran.r-project.org/web/ packages/WGCNA/index.html Open source SAS v9.4 software SAS Institute Inc. v9.4 ..



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